I'M NOW AT FLORIDA GULF COAST UNIVERSITY!
faculty of the Biological Sciences Department at FGCU as
of January 1, 2005. FGCU is
located in Fort Myers in South Florida. It is the newest campus
the University of Florida system; it opened its doors in the late
90s. FGCU is a primarily undergraduate institution; however,
M.S. and Ph.D. programs are planned. I am
continuing my research in plant-microbe interactions, with an
increasing focus on the
systems biology aspects of the work. However, I am also branching
out into new areas.
CONTINUING RESEARCH INTERESTS:
focus of my program has been signal transduction in plant disease
resistance. Research used principally bacterial pathogens and
Arabidopsis as a model system. Specific focus was in two
areas: 1. Control of programmed cell death in Arabidopsis
disease resistance; 2. Genetic circuitry, feedback regulation,
pathway cross-talk and control of informational flux in disease
resistance signaling and development of quantitative assays and
mathematical modeling techniques to underlie these studies. Close
collaboration with chemical engineering
colleagues underlied our "Systems biology" approaches. We
developed a website
that highlights these approaches and includes all
of our published data and code in multiple forms that we thought would
be useful to other systems biologists. This site does not yet
include any results from our 2004/2006 efforts, but it will be updated
our in-preparation manuscript is accepted.
FGCU, focus on computational systems biology approaches will
increase. A manuscript currently in preparation will extend our
computational models to
address cell-to-cell signaling in the Arabidopsis hypersensitive
response and report some very interesting results that clarify how the
contributes to disease resistance. My engineering
collaborators and I are also writing an undergraduate systems
textbook and beginning to develop new ways of modeling qualitative
biological data. As all models must be tested, I expect to
continue the experimental biology also, as guided by the
modeling. The only experimental work from my U. of Delaware days
that has not yet been published, aside
from a few experiments that were necessary to support the modeling work
that will be included in our "in preparation" paper, are the
results of our genetic screen for mutants affecting the HR. I'm
hoping to bring that work to the point of the first publication within
my first years at FGCU. Efforts to move what we've learned from
Arabidopsis into important crop plant systems are also underway, albeit
in early stages.
NEW RESEARCH INTERESTS:
Thanks to the tremendous talents of our Research
director, Randy Alberte, FGCU now has
funding from numerous nonconventional sources in many different
areas. My efforts thus far have been on algal biology
projects. These are just getting off the ground--watch this site
for future developments.
I taught Biochemistry (BCH 3023C) in Spring '05, Issues in Science and
Technology (IDS 3303, an upper-division core class required for all
majors in the
College of Arts and Sciences; I taught it with the topic of Genetically
Modified Organisms) in Fall '05, and Biology I Lab (BSC 101C) in Spring
Agrawal, V., Sunnam, B., Czymmek, K.J., Ogunnaike, B., Rengaswamy, R., Dhurjati, P.S. and Shapiro, A.D. (2006) Computational Modeling Explains the Trade-off Between Kinetics of Programmed Cell Death and Extent of Cell-to-Cell Signaling in Arabidopsis Defense Responses. Manuscript in preparation.
Shapiro A.D. (2006) Nitric Oxide in Cell-to-Cell Communication Coordinating the Plant Hypersensitive Response. In: Lamattina L., Polacco J.C. (ed) Nitric Oxide in Plant Growth, Development and Stress Physiology. Springer-Verlag, Berlin, Germany. In press.
Shapiro A.D., Agrawal V., Dhurjati P.S.,
Czymmek K.J., Ogunnaike B.A., Zhang
C. (2006) Systems Biology Explanations of Cell-to-Cell Communication
Coordinating the Arabidopsis Hypersensitive Response. In: Sanchez F.,
Geiger O. (ed) Biology of
Plant-Microbe Interactions, Vol. 5.
International Society for Molecular Plant-Microbe Interactions, St.
Paul, Minnesota. In press.
Shapiro, A.D. (2005) Nitric Oxide Signaling in Plants. Vitamins and
339-398. This Special Issue was also published as a book
Hormones", edited by Gerald Litwack.
Gutsche, A.T. and Shapiro, A.D.
Feedback Control of the
Arabidopsis Hypersensitive Response. Molecular
Interactions 17: 357-365.
Agrawal, V., Zhang, C., Shapiro, A.D. and Dhurjati, P.S. (2004) A Dynamic Mathematical Model to Clarify Signaling Circuitry Underlying Programmed Cell Death Control in Arabidopsis Disease Resistance. Biotechnology Progress 20: 426-442.
Zhang, C., Czymmek, K.J. and Shapiro, A.D. (2003) Nitric Oxide Does Not Trigger Early Programed Cell Death Events but May Contribute to Cell-to-Cell Signaling Governing Progression of the Arabidopsis Hypersensitive Response. Molecular Plant-Microbe Interactions 16: 962-972.
Shapiro, A.D. and Gutsche, A.T. (2003) Capillary Electrophoresis-based Profiling and Quantitation of Total Salicylic Acid and Related Phenolics for Analysis of Early Signaling in Arabidopsis Disease Resistance. Analytical Biochemistry 320: 223-233.
Zhang, C. and Shapiro, A.D. (2002) Two Pathways Act in an Additive Rather than Obligatorily Synergistic Fashion to Induce Systemic Acquired Resistance and PR Gene Expression. BioMedCentral Plant Biology 2:9.
Shapiro, A. D. (2000) Using Arabidopsis
Mutants to Delineate Disease Resistance Signaling Pathways. Canadian
Journal of Plant Pathology 22: 199-216.
Century, K. S., Shapiro, A. D., Repetti, P. P., Dahlbeck, D., Holub, E. and Staskawicz, B. J. (1997) NDR1: A Pathogen-Induced Component Required for Arabidopsis Disease Resistance. Science 278: 1963-1965.
Florida Gulf Coast University
10501 FGCU Blvd. South
Ft. Myers, FL 33965
WHAT HAPPENED TO MY DELAWARE LAB PERSONNEL?
My first Ph.D.
student, Chu Zhang, started in the
lab September 1998 and defended her thesis in September 2003. She is
now a postdoc with Dr. Cindy Carson in the Biological Sciences Dept. of
the University of Delaware, working to understand the
basis of metastatic prostate cancer.
My second Ph.D. student, Vikas Agrawal, started with us in August
2001. He defended his Ph.D. in August 2004 and moved on to a
appointment with Dr. Elliot Meyerowitz (Cal Tech). He
subsequently moved on to a job with Intel Corporation. My
fellow Dr. Cathy Worley (January 1999 - June 2001), who played the
leading role in the genetic screens we did, went on to become a
manager with Dade-Berhing Corporation in Glasgow, Delaware, working
with medical diagnostics. She then went on to work as a patent
examiner in the US Patent Office. My postdoctoral fellow Raghavan
Ramanathan (August 2000 - August 2001), who worked on early aspects of
our computational modeling, returned to his orginal field of
computational fluid dynamics in a second postdoc at U. Penn and then
returned to India. Former visiting scholar Dr. Anita Brinker
(February 2000 - October 2000) left to take a permanent position
working with Dr. Ilya Raskin at Rutgers. Former visiting scholar Yang
Hong-yu (January 2001 - September 2001) returned to her Associate
Professor position at Kunming University in China. My technician
Barb Farnworth (August 2000 - September 2002) returned to her native
Canada to a job with Response Biomedical Corporation. My original
technician Cindy Boettger
1997 - June 2000) now works for John Doms in the Animal Sciences
Department. Two other graduate students initiated degree programs
but left for personal reasons. Both of them (Thaya Ganzke and
Revital Herrmann) are currently working at DuPont.
Created January 2005
Last updated February 28, 2006
Copyright © Florida Gulf Coast University, 2005.